| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Giannotti, Sienna (12)
|
MATWAC |
31.26 |
32.22 |
0.96 |
| 2 |
Kenderdine, Skylar P (10)
|
MATWAC |
34.94 |
33.24 |
-1.70 |
| 3 |
McKendrick, Ainsley K (10)
|
MATWAC |
35.76 |
33.88 |
-1.88 |
| 4 |
Guerrero, Priscilla N (11)
|
MATWAC |
36.09 |
33.94 |
-2.15 |
| 5 |
Lagares, Addison (11)
|
MATWAC |
40.45 |
38.24 |
-2.21 |
| 6 |
Steurer, Mackenzie R (13)
|
MATWAC |
39.68 |
38.96 |
-0.72 |
| 7 |
Jansen, Sophia A (9)
|
MATWAC |
|
40.55 |
- |
| 8 |
Hariegel, Caetlin A (12)
|
MATWAC |
|
41.28 |
- |
| 9 |
Chen, Joanna (12)
|
MATWAC |
44.79 |
42.48 |
-2.31 |
| 10 |
Stankoski, Norah R (10)
|
MAUN |
43.14 |
43.65 |
0.51 |
| 11 |
Williams, Nia R (10)
|
MATWAC |
|
47.94 |
- |
| 12 |
McShane, Sadie R (10)
|
MATWAC |
|
51.23 |
- |
| 13 |
Morrison, Mary C (10)
|
MATWAC |
|
51.29 |
- |
| 14 |
Glackin, Evangelina M (9)
|
MATWAC |
|
54.61 |
- |
| 15 |
Hunter, Claire E (9)
|
MATWAC |
|
1:07.31 |
- |
|
Grasso, Reese G (11)
|
MATWAC |
|
NS |
- |
|
Brady, Leah C (10)
|
MATWAC |
39.05 |
NS |
- |